Which enzyme is essential for the removal of RNA primers during DNA replication?

The enzyme that is crucial for the removal of RNA primers during DNA replication is DNA polymerase I. This enzyme plays a key role in the replication process by synthesizing DNA and ensuring that any RNA primers laid down during the initiation of replication are replaced with DNA.

In the context of DNA replication, RNA primers serve as starting points for DNA synthesis since DNA polymerases cannot initiate synthesis without a primer. Once the DNA strands are elongated, DNA polymerase I is responsible for detecting these RNA primers and excising them. After removing the RNA primers, DNA polymerase I fills in the resulting gaps with DNA nucleotides, ensuring the integrity and continuity of the new DNA strand.

This process is essential for maintaining the accuracy and consistency of DNA replication, as any leftover RNA sequences could lead to genetic instability. Understanding the specific roles of these enzymes helps clarify their contributions to the overall mechanism of DNA replication.